PINK1 antibody (C-Term)

Catalog No. ABIN7540590

Supplier Product No.: 600-401-gu5s

The Rabbit Polyclonal anti-PINK1 antibody has been validated for WB, IHC, ELISA, IF, IP, FACS and FM. It is suitable to detect PINK1 in samples from Human. There is 1 publication available.

Quick Overview for PINK1 antibody (C-Term) (ABIN7540590)

Target: PINK1 (PTEN Induced Putative Kinase 1 (PINK1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PINK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Product Details anti-PINK1 Antibody

Binding Specificity: C-Term

Supplier Product No.: 600-401-gu5s

Supplier: Rockland

Purpose: PINK1 truncated Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against human PINK1.

Purification: The antibody was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen: Anti-PINK1 truncated antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Term portion of human PINK1 conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes:

Immunoprecipitation_Dilution: User Optimized

ELISA_Dilution: User Optimized

Immunohistochemistry_Dilution: 1:200

Flow_Cytometry_Dilution: User Optimized

IF_Microscopy_Dilution: 20 μg/mL

Western_Blot_Dilution: 1 μg/mL

Comment:

Suggested Applications: FC
Anti-PINK1 truncated Antibody has been tested in Western Blot, Immunofluorescence, and ELISA. Positive controls were recombinant PINK1 protein and overexpressed PINK1 transfected HEK293 @ 1μg/mL in western blot. Expect a lower strength band at 62.7 kDa in western blot using appropriate lysates. PINK1 truncated antibody strongly detects endogenously truncated PINK1 protein, seen at ~30kDa. PINK1 is highly phosphorylated and degrades rapidly which may cause additional banding in western blot. Positive control cell lines for immunofluorescence assays are NIH3T3 (p/n W10-000-358). Although not tested, this antibody is likely functional in immunohistochemistry, flow cytometry, and immunoprecipitation.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

References for anti-PINK1 antibody (ABIN7540590)

Chen, Katsuyama, Satyam, Li, Rubio, Jung, Andrzejewski, Becherer, Tsokos, Abdi, Tsokos: "CD38 reduces mitochondrial fitness and cytotoxic T cell response against viral infection in lupus patients by suppressing mitophagy." in: Science advances, Vol. 8, Issue 24, pp. eabo4271, (2022) (PubMed).

Target Details for PINK1

Target: PINK1 (PTEN Induced Putative Kinase 1 (PINK1))

Alternative Name: PINK1

Background: Rabbit anti-PINK1 antibody, Serine/threonine-protein kinase PINK1 (mitochondrial), BRPK, PTEN-induced putative kinase protein 1, PTEN-induced kinase,PINK1 (PTEN induced putative kinase 1) belongs to the protein kinase superfamily and Ser/Thr protein kinase family. PINK1 protects against mitochondrial dysfunction during cellular stress by phosphorylating mitochondrial proteins. It is involved in the clearance of damaged mitochondria via selective autophagy (mitophagy) by mediating activation and translocation of PARK2. Targets PARK2 to dysfunctional depolarized mitochondria through the phosphorylation of MFN2. Activates PARK2 in 2 steps: (1) by mediating phosphorylation at Ser-65 of PARK2 and (2) mediating phosphorylation of ubiquitin, converting PARK2 to its fully-active form. PINK1 interacts with PARK2 and FBXO7 and forms a complexes with PARK2 and PARK7. PINK1 is associated with Parkinson disease 6 (PARK6). Anti-PINK1 Antibody is useful for researcher interested in neurological research, neurodegenerative disorders, Pink/Parkin Mediated Mitophagy, and Parkinsons Disease Pathway.

Gene ID: 65018

NCBI Accession: NP_115785

UniProt: Q9BXM7

Pathways: Autophagy